parseCIRCexplorer
parseCIRCExplorer takes the identified circRNA results from
CIRCexplorer and save as a
GVF file. The GVF file can be later used to call variant peptides using
callVariant. Noted that only known circRNA is supported (
*_circular_known.txt)
Reference Version
The version of reference genome and proteome FASTA and annotation GTF MUST be consistent across all analysis.
Usage
usage: moPepGen parseCIRCexplorer [-h] -i <file> -o <file> [--circexplorer3]
[--min-read-number <number>]
[--min-fpb-circ <number>]
[--min-circ-score <number>]
[--intron-start-range <number>]
[--intron-end-range <number>]
[--skip-failed] --source SOURCE [-a <file>]
[--reference-source {GENCODE,ENSEMBL}]
[--codon-table {SGC5,Hexamita Nuclear,Euplotid Nuclear,Ascidian Mitochondrial,Thraustochytrium Mitochondrial,Standard,Gracilibacteria,SGC8,Condylostoma Nuclear,Flatworm Mitochondrial,SGC1,Protozoan Mitochondrial,SGC3,Pachysolen tannophilus Nuclear,Archaeal,Chlorophycean Mitochondrial,Pterobranchia Mitochondrial,Echinoderm Mitochondrial,Blastocrithidia Nuclear,Invertebrate Mitochondrial,SGC0,Mycoplasma,Yeast Mitochondrial,Mesodinium Nuclear,Vertebrate Mitochondrial,Bacterial,Trematode Mitochondrial,SGC4,Balanophoraceae Plastid,Blepharisma Macronuclear,Mold Mitochondrial,Karyorelict Nuclear,Spiroplasma,SGC9,Scenedesmus obliquus Mitochondrial,Plant Plastid,Coelenterate Mitochondrial,Alternative Yeast Nuclear,Dasycladacean Nuclear,Candidate Division SR1,SGC2,Cephalodiscidae Mitochondrial,Peritrich Nuclear,Ciliate Nuclear,Alternative Flatworm Mitochondrial}]
[--chr-codon-table [CHR_CODON_TABLE [CHR_CODON_TABLE ...]]]
[--start-codons [START_CODONS [START_CODONS ...]]]
[--chr-start-codons [CHR_START_CODONS [CHR_START_CODONS ...]]]
[--index-dir [<file>]]
[--debug-level <value|number>] [-q]
Parse CIRCexplorer result to a TSV format for moPepGen to call variant
peptides
optional arguments:
-h, --help show this help message and exit
-i <file>, --input-path <file>
File path to CIRCexplorer's TSV output. Valid formats:
['.tsv', '.txt'] (default: None)
-o <file>, --output-path <file>
File path to the output file. Valid formats: ['.gvf']
(default: None)
--circexplorer3 Using circRNA resutls from CIRCexplorer3 (default:
False)
--min-read-number <number>
Minimal number of junction read counts. (default: 1)
--min-fpb-circ <number>
Minimal CRICscore value for CIRCexplorer3. Recommends
to 1, defaults to None (default: None)
--min-circ-score <number>
Minimal CIRCscore value for CIRCexplorer3. Recommends
to 1, defaults to None (default: None)
--intron-start-range <number>
The range of difference allowed between the intron
start and the reference position. (default: -2,0)
--intron-end-range <number>
The range of difference allowed between the intron end
and the reference position. (default: -100,5)
--skip-failed When set, the failed records will be skipped.
(default: False)
--source SOURCE Variant source (e.g. gSNP, sSNV, Fusion) (default:
None)
--debug-level <value|number>
Debug level. (default: INFO)
-q, --quiet Quiet (default: False)
Reference Files:
-a <file>, --annotation-gtf <file>
Path to the annotation GTF file. Only ENSEMBL and
GENCODE are supported. Its version must be the same as
the genome and proteome FASTA. (default: None)
--reference-source {GENCODE,ENSEMBL}
Source of reference genome and annotation. (default:
None)
--codon-table {SGC5,Hexamita Nuclear,Euplotid Nuclear,Ascidian Mitochondrial,Thraustochytrium Mitochondrial,Standard,Gracilibacteria,SGC8,Condylostoma Nuclear,Flatworm Mitochondrial,SGC1,Protozoan Mitochondrial,SGC3,Pachysolen tannophilus Nuclear,Archaeal,Chlorophycean Mitochondrial,Pterobranchia Mitochondrial,Echinoderm Mitochondrial,Blastocrithidia Nuclear,Invertebrate Mitochondrial,SGC0,Mycoplasma,Yeast Mitochondrial,Mesodinium Nuclear,Vertebrate Mitochondrial,Bacterial,Trematode Mitochondrial,SGC4,Balanophoraceae Plastid,Blepharisma Macronuclear,Mold Mitochondrial,Karyorelict Nuclear,Spiroplasma,SGC9,Scenedesmus obliquus Mitochondrial,Plant Plastid,Coelenterate Mitochondrial,Alternative Yeast Nuclear,Dasycladacean Nuclear,Candidate Division SR1,SGC2,Cephalodiscidae Mitochondrial,Peritrich Nuclear,Ciliate Nuclear,Alternative Flatworm Mitochondrial}
Codon table. Defaults to "Standard". Supported codon
tables: {'SGC5', 'Hexamita Nuclear', 'Euplotid
Nuclear', 'Ascidian Mitochondrial', 'Thraustochytrium
Mitochondrial', 'Standard', 'Gracilibacteria', 'SGC8',
'Condylostoma Nuclear', 'Flatworm Mitochondrial',
'SGC1', 'Protozoan Mitochondrial', 'SGC3', 'Pachysolen
tannophilus Nuclear', 'Archaeal', 'Chlorophycean
Mitochondrial', 'Pterobranchia Mitochondrial',
'Echinoderm Mitochondrial', 'Blastocrithidia Nuclear',
'Invertebrate Mitochondrial', 'SGC0', 'Mycoplasma',
'Yeast Mitochondrial', 'Mesodinium Nuclear',
'Vertebrate Mitochondrial', 'Bacterial', 'Trematode
Mitochondrial', 'SGC4', 'Balanophoraceae Plastid',
'Blepharisma Macronuclear', 'Mold Mitochondrial',
'Karyorelict Nuclear', 'Spiroplasma', 'SGC9',
'Scenedesmus obliquus Mitochondrial', 'Plant Plastid',
'Coelenterate Mitochondrial', 'Alternative Yeast
Nuclear', 'Dasycladacean Nuclear', 'Candidate Division
SR1', 'SGC2', 'Cephalodiscidae Mitochondrial',
'Peritrich Nuclear', 'Ciliate Nuclear', 'Alternative
Flatworm Mitochondrial'} (default: Standard)
--chr-codon-table [CHR_CODON_TABLE [CHR_CODON_TABLE ...]]
Chromosome specific codon table. Must be specified in
the format of "chrM:SGC1", where "chrM" is the
chromosome name and "SGC1" is the codon table to use
to translate genes on chrM. Supported codon tables:
{'SGC5', 'Hexamita Nuclear', 'Euplotid Nuclear',
'Ascidian Mitochondrial', 'Thraustochytrium
Mitochondrial', 'Standard', 'Gracilibacteria', 'SGC8',
'Condylostoma Nuclear', 'Flatworm Mitochondrial',
'SGC1', 'Protozoan Mitochondrial', 'SGC3', 'Pachysolen
tannophilus Nuclear', 'Archaeal', 'Chlorophycean
Mitochondrial', 'Pterobranchia Mitochondrial',
'Echinoderm Mitochondrial', 'Blastocrithidia Nuclear',
'Invertebrate Mitochondrial', 'SGC0', 'Mycoplasma',
'Yeast Mitochondrial', 'Mesodinium Nuclear',
'Vertebrate Mitochondrial', 'Bacterial', 'Trematode
Mitochondrial', 'SGC4', 'Balanophoraceae Plastid',
'Blepharisma Macronuclear', 'Mold Mitochondrial',
'Karyorelict Nuclear', 'Spiroplasma', 'SGC9',
'Scenedesmus obliquus Mitochondrial', 'Plant Plastid',
'Coelenterate Mitochondrial', 'Alternative Yeast
Nuclear', 'Dasycladacean Nuclear', 'Candidate Division
SR1', 'SGC2', 'Cephalodiscidae Mitochondrial',
'Peritrich Nuclear', 'Ciliate Nuclear', 'Alternative
Flatworm Mitochondrial'}. By default, "SGC1" is
assigned to mitochondrial chromosomes. (default: [])
--start-codons [START_CODONS [START_CODONS ...]]
Default start codon(s) to use for novel ORF
translation. Defaults to ["ATG"]. (default: ['ATG'])
--chr-start-codons [CHR_START_CODONS [CHR_START_CODONS ...]]
Chromosome specific start codon(s). For example,
"chrM:ATG,ATA,ATT".By defualt, mitochondrial
chromosome name is automatically inferred andstart
codon "ATG", "ATA", "ATT", "ATC" and "GTG" are
assigned to it. (default: [])
--index-dir [<file>] Path to the directory of index files generated by
moPepGen generateIndex. If given, --genome-fasta,
--proteome-fasta and --anntotation-gtf will be
ignored. (default: None)
Arguments
-h, --help
show this help message and exit
-i, --input-path <file> Path
File path to CIRCexplorer's TSV output. Valid formats: ['.tsv', '.txt']
-o, --output-path <file> Path
File path to the output file. Valid formats: ['.gvf']
--circexplorer3
Using circRNA resutls from CIRCexplorer3
Default: False
--min-read-number <number> int
Minimal number of junction read counts.
int
Default: 1
--min-fpb-circ <number> float
Minimal CRICscore value for CIRCexplorer3. Recommends to 1, defaults to None
--min-circ-score <number> float
Minimal CIRCscore value for CIRCexplorer3. Recommends to 1, defaults to None
--intron-start-range <number> str
The range of difference allowed between the intron start and the reference position.
str
Default: -2,0
--intron-end-range <number> str
The range of difference allowed between the intron end and the reference position.
str
Default: -100,5
--skip-failed
When set, the failed records will be skipped.
Default: False
--source str
Variant source (e.g. gSNP, sSNV, Fusion)
-a, --annotation-gtf <file> Path
Path to the annotation GTF file. Only ENSEMBL and GENCODE are supported. Its version must be the same as the genome and proteome FASTA.
--reference-source str
Source of reference genome and annotation.
Choices: ['GENCODE', 'ENSEMBL']
--codon-table str
Codon table. Defaults to "Standard". Supported codon tables: {'SGC5', 'Hexamita Nuclear', 'Euplotid Nuclear', 'Ascidian Mitochondrial', 'Thraustochytrium Mitochondrial', 'Standard', 'Gracilibacteria', 'SGC8', 'Condylostoma Nuclear', 'Flatworm Mitochondrial', 'SGC1', 'Protozoan Mitochondrial', 'SGC3', 'Pachysolen tannophilus Nuclear', 'Archaeal', 'Chlorophycean Mitochondrial', 'Pterobranchia Mitochondrial', 'Echinoderm Mitochondrial', 'Blastocrithidia Nuclear', 'Invertebrate Mitochondrial', 'SGC0', 'Mycoplasma', 'Yeast Mitochondrial', 'Mesodinium Nuclear', 'Vertebrate Mitochondrial', 'Bacterial', 'Trematode Mitochondrial', 'SGC4', 'Balanophoraceae Plastid', 'Blepharisma Macronuclear', 'Mold Mitochondrial', 'Karyorelict Nuclear', 'Spiroplasma', 'SGC9', 'Scenedesmus obliquus Mitochondrial', 'Plant Plastid', 'Coelenterate Mitochondrial', 'Alternative Yeast Nuclear', 'Dasycladacean Nuclear', 'Candidate Division SR1', 'SGC2', 'Cephalodiscidae Mitochondrial', 'Peritrich Nuclear', 'Ciliate Nuclear', 'Alternative Flatworm Mitochondrial'}
str
Default: Standard
Choices: {'SGC5', 'Hexamita Nuclear', 'Euplotid Nuclear', 'Ascidian Mitochondrial', 'Thraustochytrium Mitochondrial', 'Standard', 'Gracilibacteria', 'SGC8', 'Condylostoma Nuclear', 'Flatworm Mitochondrial', 'SGC1', 'Protozoan Mitochondrial', 'SGC3', 'Pachysolen tannophilus Nuclear', 'Archaeal', 'Chlorophycean Mitochondrial', 'Pterobranchia Mitochondrial', 'Echinoderm Mitochondrial', 'Blastocrithidia Nuclear', 'Invertebrate Mitochondrial', 'SGC0', 'Mycoplasma', 'Yeast Mitochondrial', 'Mesodinium Nuclear', 'Vertebrate Mitochondrial', 'Bacterial', 'Trematode Mitochondrial', 'SGC4', 'Balanophoraceae Plastid', 'Blepharisma Macronuclear', 'Mold Mitochondrial', 'Karyorelict Nuclear', 'Spiroplasma', 'SGC9', 'Scenedesmus obliquus Mitochondrial', 'Plant Plastid', 'Coelenterate Mitochondrial', 'Alternative Yeast Nuclear', 'Dasycladacean Nuclear', 'Candidate Division SR1', 'SGC2', 'Cephalodiscidae Mitochondrial', 'Peritrich Nuclear', 'Ciliate Nuclear', 'Alternative Flatworm Mitochondrial'}
--chr-codon-table str
Chromosome specific codon table. Must be specified in the format of "chrM:SGC1", where "chrM" is the chromosome name and "SGC1" is the codon table to use to translate genes on chrM. Supported codon tables: {'SGC5', 'Hexamita Nuclear', 'Euplotid Nuclear', 'Ascidian Mitochondrial', 'Thraustochytrium Mitochondrial', 'Standard', 'Gracilibacteria', 'SGC8', 'Condylostoma Nuclear', 'Flatworm Mitochondrial', 'SGC1', 'Protozoan Mitochondrial', 'SGC3', 'Pachysolen tannophilus Nuclear', 'Archaeal', 'Chlorophycean Mitochondrial', 'Pterobranchia Mitochondrial', 'Echinoderm Mitochondrial', 'Blastocrithidia Nuclear', 'Invertebrate Mitochondrial', 'SGC0', 'Mycoplasma', 'Yeast Mitochondrial', 'Mesodinium Nuclear', 'Vertebrate Mitochondrial', 'Bacterial', 'Trematode Mitochondrial', 'SGC4', 'Balanophoraceae Plastid', 'Blepharisma Macronuclear', 'Mold Mitochondrial', 'Karyorelict Nuclear', 'Spiroplasma', 'SGC9', 'Scenedesmus obliquus Mitochondrial', 'Plant Plastid', 'Coelenterate Mitochondrial', 'Alternative Yeast Nuclear', 'Dasycladacean Nuclear', 'Candidate Division SR1', 'SGC2', 'Cephalodiscidae Mitochondrial', 'Peritrich Nuclear', 'Ciliate Nuclear', 'Alternative Flatworm Mitochondrial'}. By default, "SGC1" is assigned to mitochondrial chromosomes.
str
Default: []
--start-codons str
Default start codon(s) to use for novel ORF translation. Defaults to ["ATG"].
str
Default: ['ATG']
--chr-start-codons str
Chromosome specific start codon(s). For example, "chrM:ATG,ATA,ATT".By defualt, mitochondrial chromosome name is automatically inferred andstart codon "ATG", "ATA", "ATT", "ATC" and "GTG" are assigned to it.
str
Default: []
--index-dir <file> Path
Path to the directory of index files generated by moPepGen generateIndex. If given, --genome-fasta, --proteome-fasta and --anntotation-gtf will be ignored.
--debug-level <value|number> str
Debug level.
str
Default: INFO
-q, --quiet
Quiet
Default: False